Published by Informa UK Limited, trading as Taylor & Francis Group. Sheep morphometry rumen stereology surfaces volumes. The methods documented here provide the possibility of doing a detailed stereological analysis of ruminal tissue in different experimental or even pathological conditions. Average absolute surface area was 4726.74 ± 628.56 cm 2. stereology: noun a branch of science concerned with inferring the three-dimensional properties of objects or matter ordinarily observed two-dimensionally. The volume of ruminal tissue was 536.54 ± 80.52 cm 3, the macroscopic surface area was 1091 ± 115.75 cm 2 with a papillary packing density of 84.64 ± 10.99 cm -2. Papillary densities and numbers were estimated from horizontal sections. Tissue blocks for histology were obtained by systematic random sampling and processed to obtain vertical sections for surface area and volume estimations. Volumes of the ruminal tissues were estimated by the volume displacement method, while volume densities of the components of the ruminal wall were estimated by point counting methods. Macroscopic ruminal surface area was estimated using the point-associated area method. The sheep were euthanized with 20% sodium pentobarbital intravenously, the rumen was dissected out and divided into the various sacs for gross examination, and fixation by total immersion in 10% formalin. What does stereological mean Information and translations of stereological in the most comprehensive dictionary definitions resource on the web. Make an order anytime, knowing that competent, trustworthy writers will be doing your essay. Definition of stereological in the dictionary. Thus stereology provides biologically meaningful 3D data. Unbiased Stereology: A Practical GuideV payments, and your personal data are totally safe with us. In the current investigation, five rams were used to demonstrate the methods for quantifying salient structural parameters related to rumen function. Practical stereology is the application of unbiased sampling and measurement principles to obtain quantitative data about structures in 3D based on nearly 2D (physical or virtual) sections through the structures by using 3D (or geometric) probes.
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Stereological analysis of important structural parameters that may be used to assess the functional capacity of the rumen is lacking. In ruminants, the rumen is the largest and most significant fore-stomach.